The W.H. OvulTell Test is an immunochromatographic one step assay designed for in vitro qualitative determination of human luteinizing hormone (hLH) in urine to predict time of ovulation. LH surge usually comes before ovulation, which means release of an egg. An egg can only live for 24 hours or less after it is released, while sperm survives only a few days in your body. Consequently, the W.H. OvulTell Test can help you predict the two most fertile days following the detected LH surge. Having intercourse within these days will maximize your chances of becoming pregnant.

REAGENTS AND MATERIALS

Each W.H. OvulTell Test midstream is individually sealed in a foil pouch.

Materials Required But Not Provided: Timer, specimen container.

STORAGE AND STABILITY

The test kit is to be stored at room temperature in the sealed pouch for the duration of the shelf life.

PRECAUTION

Do not use the test kit beyond the expiry date.

WHEN TO START TESTING

First, determine the average length of your menstrual cycle. The length of menstrual cycle is the number of days from the first day of menstrual bleeding to the day before bleeding begins on the next period. Determine the usual length of the menstrual cycle over the last few months. Then refer to the Cycle Chart to determine on which day of the menstrual cycle to begin testing.

Cycle Chart

Usual Cycle Length	Days to begin Testing
21	05
22	06
23	07
24	08
25	09
26	10
27	11
28	12
29	13
30	14
31	15
32	16
33	17
34	18
35	19
36	20
37	21
38	22
39	23
40	24

For example, if your usual cycle is 28 days. If your first day of last menstruation is 7^th, you should begin testing on day 18^th.

Example Calendar

Sun.	Mon.	Tue.	Wed.	Thu.	Fri.	Sat.
	1	2	3	4	5	6
(7)	8	9	10	11	12	13
14	15	16	17	<18>	19	20
21	22	23	24	25	26	27
28	29	30	31

( )--First day of your last period

< >-- Begin testing with the W.H. OvulTell One Step Ovulation Test (Urine).

ASSAY PROCEDURE

Choose a convenient time of the day to perform the test. Try to test at about the same time each day for the entire cycle. For best results, test between 10:00 am and 8:00 pm .

1. Remove the midstream test from the foil pouch.

2. Remove the Cap.

3.Hold the midstream test by the Thumb Grip with the exposed Absorbent Tip pointing downward directly into your urine stream for at least 10 seconds until it is thoroughly wet.

4. Note: Do not urinate on the Test and Control windows. If you prefer, you can urinate into a clean and dry container, then dip only the Absorbent Tip of the midstream test into the urine for at least 10 seconds.

5.After removing the midstream test from your urine, immediately replace the Cap over the Absorbent Tip, lay the midstream test on a flat surface with the Test and Control windows facing upwards, and then begin timing.

6.Read the results at 5minutes.

7. DO NOT INTERPRET RESULT AFTER 10 MINUTES.

INTERPRETATION OF RESULTS ' (The Test (T ) window shape may vary slightly. The Control (C) window is always a circle)

1. Surge: If the surge band (T) is more intensive than or equivalent to that of reference band (C), the specimen is likely at or around the surge level.

2. No Surge: If the colour intensity of the surge band (T) is less than that of reference band (C), the specimen is likely at its basal LH levels.

3. Invalid: When neither surge band (T) or reference band (C) appears on the membrane, the test should be voided since improper test procedure or deterioration of reagents probably occurred

SURGE	NO SURGE	INVALID